Ngamaxesha e-COVID-19 uhlalutyo lweeseli ze-peripheral ze-mononuclear zegazi (PBMCs) kunye neepateni zazo ze-CD-marker yimilinganiselo eyimfuneko ehambisa idatha ebalulekileyo ukuqonda ngcono ukuqhubeka kosulelo yi-SARS-CoV-2 ebantwini.
Ngokuqhelekileyo uhlalutyo lwe-PBMC lweesampulu zegazi elipheleleyo kudla ixesha
inkqubo.I-Countstar Rigel inciphisa eli xesha lokuhlalutya ngokuphawulekayo ngokusebenzisa indlela ye-AO / PI staining.Isoftware yesixhobo inciphisa ukubala okuxhaphakileyo kunye namanyathelo kuhlalutyo olongezelelweyo (i-diameter / aggregation rate).
I-Countstar Rigel inikezela ngeziphumo ezichanekileyo kunye nezithelekisekayo ukongeza kwimifanekiso ephezulu ye-CD4 + iiseli ngokukhawuleza kunendlela ye-cytometry yokuhamba yendabuko.Ngaphaya koko, abahlalutyi be-Countstar Rigel sele bebonakalise ukuchaneka kwabo kunye nokuveliswa kwakhona kwiinkqubo ezininzi ze-cGMP ezilawulwayo zokwenziwa kogonyo kunye nezithako ezisebenzayo zamayeza (APIs) kwihlabathi liphela.
Buza iqabane lakho lokuthengisa lengingqi okanye uqhagamshelane nathi ngokuthe ngqo ukucwangcisa idemo okanye uvavanyo lweemodeli ze-Countstar Rigel.Iingcali zethu zezicelo zikulungele ukukunceda kwintshayelelo kunye noqeqesho.
Umzobo 1
Icandelo lomfanekiso wentsimi oqaqambileyo, ofunyenwe kwisampulu ye-PBMC yegazi lonke yi-Countstar Rigel S3, iqulethe i-debris eninzi, iiplatelet kunye nezinye izinto ezingachazwanga.
Umzobo 2
Umfanekiso ogqithisiweyo, icandelo elifanayo, iiseli ezingcoliswe yi-AO / PI, i-Channel 1 (Ex / Em 480nm / 535/40nm) I-Channel 2 (Ex / Em: 525nm / 580/25nm : Ebomvu: iseli efileyo, eluhlaza: iseli esebenzayo, i-Orange: into engabhalwanga, engachazwanga
Umzobo 3
Idatha ye-cytometry ehambayo xa kuthelekiswa neziphumo ze-Countstar Rigel, ukulinganisa i-CD3-FITC kunye ne-CD4-PE yokubhala iiseli zomzimba ezivuselelwe yi-IL-6
