Kurapa kwesero pasina mubvunzo tariro itsva yekutungamira ramangwana rebiomedicine, asi kushandiswa kwemasero evanhu mumishonga haisi pfungwa itsva.Mumakumi mashoma emakore apfuura, kurapa kwesero kwave kufambira mberi, uye kurapa kwesero pachayo hakuchisiri muunganidzwa wakapfava wemaseru uye kudzoserwa kumashure.Masero zvino anowanzo kudikanwa kuti ave bioengineered, akadai seCAR-T cell therapy.Isu tinovavarira kukupa yakamisikidzwa, GMP nhanho yemidziyo yekudzora mhando yesero.Chigadzirwa che Countstar chakagamuchirwa nemakambani mazhinji anotungamira sero therapy, isu tinogona kubatsira mutengi wedu kuvaka yakagadzikana, yakavimbika sero rekuisa, viability monitor system.
Dambudziko muSero kuverenga uye kugona
Pakati pematanho ese ekiriniki yeCAR-T masero ekugadzira, kugona uye kuverenga kwesero kunofanirwa kutariswa chaizvo.
Maseru achangozvipatsanurwa ekutanga kana maseru ane tsika anogona kunge aine tsvina, akati wandei marudzi emasero kana twunhu tunovhiringidza senge marara emasero izvo zvinozoita kuti zvisaite kuongorora maseru ekufarira.
Dual Fluorescence viability kuverenga na Countstar Rigel S2
Acridine orenji (AO) uye Propidium iodide (PI) inyukireya nucleic acid inosunga dhayi.AO inogona kupinda kune ese akafa uye mhenyu maseru uye inosvibisa masero ane nucleated kuti abudise green fluorescence.PI inogona kusvibisa masero akafa nucleated ane membrane akakanganiswa uye kugadzira tsvuku fluorescence.Ongororo iyi haibatanidzi zvimedu zvemasero, marara uye zvimedu zvekugadzira pamwe nezviitiko zvakaderera semaplatelet, zvichipa mhedzisiro chaiyo.Mukupedzisa, iyo Countstar S2 system inogona kushandiswa kune yega nhanho yesero kugadzira maitiro.
A: AO/PI nzira inokwanisa kusiyanisa mhenyu uye kufa kwemasero, uye zvakare inogona kusabvisa kupindira.Nekuyedza masampula ekudiresi, nzira mbiri-fluorescence inoratidza mhedzisiro yakagadzikana.
Kutsunga kweT / NK Cell Mediated Cytotoxicity
Nekuisa mazita anonangwa bundu maseru neasina-chepfu, asiri-radioactive calcein AM kana transfect neGFP, tinogona kutarisa kuurayiwa kwemaseru bundu neCAR-T maseru.Nepo maseru egomarara anorarama achizonyorwa negirinhi calcein AM kana GFP, masero akafa haakwanise kuchengetedza dhayi yegirinhi.Hoechst 33342 inoshandiswa kusvibisa masero ese (ese maT masero uye bundu masero), neimwe nzira, inonangwa bundu masero anogona kusvibiswa ne membrane akasungwa calcein AM, PI inoshandiswa kusvibisa masero akafa (ese maT masero uye bundu masero).Iyi nzira yekusvibisa inobvumira kusarura kwemasero akasiyana.
Consistent Cell Counting uye Global Data Management
Dambudziko rakajairika mukuverengera kwesero mutsauko pakati pevashandisi, madhipatimendi uye masaiti.Yese Countstar analyzer inoverenga zvakafanana munzvimbo dzakasiyana kana nzvimbo yekugadzira.Izvi zvinodaro nekuti mukuita kwekutonga kwemhando, chiridzwa chega chega chinofanirwa kuverengerwa kune chakajairwa chiridzwa.
Central data bank inobvumira mushandisi kuchengeta data rese, senge chiridzwa bvunzo chirevo, sero sampuli repoti uye tester e-siginecha, yakachengeteka uye zvachose.
Mota T Cell Therapy: Tariro Itsva Yekurapa Kenza
CAR-T cell therapy pasina mubvunzo tariro itsva kutungamira ramangwana rebiomedicine yegomarara.Pakati pematanho ese ekiriniki yeCAR-T masero ekugadzira, kugona uye kuverenga kwesero kunofanirwa kutariswa chaizvo.
Iyo Countstar Rigel yakagamuchirwa nemakambani mazhinji anotungamira iyo CAR-T cell therapy, isu tinogona kubatsira mutengi wedu kuvaka yakagadzikana, yakavimbika sero yekumisikidza, viability monitor system.
