ʻO ka cell therapy he mea kānalua hou ia e alakaʻi i ka wā e hiki mai ana o ka biomedicine, akā ʻo ka hoʻohana ʻana i nā cell kanaka i ka lāʻau lapaʻau ʻaʻole ia he manaʻo hou.I nā makahiki i hala iho nei, ua holomua ka cell therapy, a ʻo ka cell therapy ʻaʻole ia he hōʻiliʻili maʻalahi o nā cell a hoʻihoʻi ʻia i hope.Pono pinepine ʻia nā kelepona e bioengineered, e like me ka CAR-T cell therapy.Manaʻo mākou e hāʻawi iā ʻoe i nā mea hana maʻamau, GMP pae no ka mālama ʻana i ka maikaʻi o ke kelepona.Ua ʻae ʻia ka huahana Countstar e nā ʻoihana he nui e alakaʻi ana i ka cell therapy, hiki iā mākou ke kōkua i kā mākou mea kūʻai aku e kūkulu i kahi kūpaʻa paʻa paʻa, hilinaʻi, ʻōnaehana nānā viability.
Paʻakikī i ka helu Cell a me ka viability
I ka wā o nā hana a pau o ka hana ʻana o ke kelepona CAR-T, pono e hoʻoholo pololei ʻia ke ola a me ka helu kelepona.
Loaʻa i nā pūnaewele mua i hoʻokaʻawale hou ʻia a i ʻole nā pūnaewele moʻomeheu i loko o nā mea haumia, nā ʻano cell a i ʻole nā mea hoʻopili e like me ka ʻōpala cell e hiki ʻole ai ke kālailai i nā cell o ka hoihoi.
ʻO ka helu ʻana i ka hiki ʻana o Dual Fluorescence e Countstar Rigel S2
ʻO ka ʻalani Acridine (AO) a me ka Propidium iodide (PI) nā mea hoʻopaʻa paʻa nukleika nukleika.Hiki i ke AO ke komo i loko o nā pūnaeʻa make a me nā pūnaewele ola a hoʻopaʻa i nā pūnaewele nucleated e hoʻoulu i ka fluorescence ʻōmaʻomaʻo.Hiki i ka PI ke hoʻomaʻemaʻe i nā sela nucleated make me nā membrane i hoʻopaʻa ʻia a hoʻoulu i ka fluorescence ʻulaʻula.Hoʻokuʻu ʻia ka ʻikepili i nā ʻāpana cell, nā ʻōpala a me nā ʻāpana artifact a me nā hanana undersized e like me nā platelets, e hāʻawi ana i kahi hopena pololei loa.I ka hopena, hiki ke hoʻohana ʻia ka ʻōnaehana Countstar S2 no kēlā me kēia pae o ke kaʻina hana kelepona.
A: Hiki i ke ʻano AO/PI ke hoʻokaʻawale pololei i ke kūlana ola a me ka make o nā cell, a hiki ke hoʻokaʻawale i ka hoʻopili.Ma ka hoʻāʻo ʻana i nā laʻana hoʻoheheʻe, hōʻike ke ʻano pālua-fluorescence i nā hopena paʻa.
ʻO ka hoʻoholoʻana o ka T / NK Cell Mediated Cytotoxicity
Ma ka hoʻopaʻa inoa ʻana i nā cell tumor target me ka non-toxic, non-radioactive calcein AM a i ʻole transfect me GFP, hiki iā mākou ke nānā i ka pepehi ʻia ʻana o nā pūnana maʻi e nā cell CAR-T.ʻOiai e hoʻopaʻa inoa ʻia nā cell cancer target e ka calcein ʻōmaʻomaʻo AM a i ʻole GFP, ʻaʻole hiki i nā mea make ke mālama i ka wai ʻōmaʻomaʻo.Hoʻohana ʻia ʻo Hoechst 33342 no ka hoʻomaʻemaʻe ʻana i nā cell āpau (ʻo nā pūnae T a me nā pūnaewele tumo), ʻo ia hoʻi, hiki ke hoʻopaʻa ʻia nā cell tumor target me ka calcein AM i hoʻopaʻa ʻia me ka membrane, hoʻohana ʻia ʻo PI no ka hoʻopaʻa ʻana i nā pūnana make (ʻelua mau pūnaewele T a me nā pūnaewele tumo).Hiki i kēia hoʻolālā kala ke hoʻokaʻawale i nā cell like ʻole.
Ka helu kelepona paʻa a me ka hoʻokele ʻikepili honua
ʻO kahi pilikia maʻamau i ka helu kelepona maʻamau ka ʻokoʻa ʻikepili ma waena o nā mea hoʻohana, nā keʻena a me nā pūnaewele.He helu like nā mea helu helu Countstar a pau ma nā wahi like ʻole a i ʻole kahua hana.ʻO kēia no ka mea ma ke kaʻina o ka mana o ka maikaʻi, pono e hoʻohālikelike ʻia kēlā me kēia mea kani i ka mea hana maʻamau.
Hāʻawi ka waihona ʻikepili kikowaena i ka mea hoʻohana e mālama i nā ʻikepili āpau, e like me ka hōʻike hōʻike mea hana, hōʻike hōʻike cell a me ka hōʻailona e-signature, palekana a mau loa.
Kaʻa T Cell Therapy: He Manaolana Hou no ka Lapaʻau ʻana i ka maʻi maʻi maʻi
ʻO ka CAR-T cell therapy he mea kānalua ia he manaʻolana hou e alakaʻi i ka wā e hiki mai ana o ka biomedicine no ka maʻi kanesa.I ka wā o nā hana a pau o ka hana ʻana o ke kelepona CAR-T, pono e hoʻoholo pololei ʻia ke ola a me ka helu kelepona.
Ua ʻae ʻia ka Countstar Rigel e nā ʻoihana he nui e alakaʻi ana i ka cell therapy CAR-T, hiki iā mākou ke kōkua i kā mākou mea kūʻai aku e kūkulu i kahi paʻa paʻa, hilinaʻi cell concentration, viability monitor system.
