Toti-N-glycan Recognition Enables Universal Multiplexed Single-Nucleus RNA Sequencing

The study introduces Toti-N-glycan recognition as a universal sample-labeling strategy for multiplexed single-nucleus RNA sequencing. By exploiting broadly available nuclear glycan features, the workflow supported accurate sample assignment across diverse species and tissues while reducing batch effects and expanding the practical reach of multiplexed single-cell analysis.

The Countstar Mira FL fluorescence cell analyzer was used during sample preparation to quantify cells or nuclei before multiplexed single-nucleus RNA sequencing.