The peripheral Atf3 + neuronal population is responsible for nerve regeneration at the early stage of nerve injury revealed by single-cell RNA sequencing.

The authors also confirm that regenerative progress occurs at the early stage of nerve injury because immunohistochemistry shows that the expression of ATF3 is significantly increased beginning at 3 days post-CCI and decreased at 1 month post-CCI.

Using Countstar (model not specified), the researchers measured cell viability in the study's cell samples before single-cell analysis for the study's single-cell analysis.