SCIENTIFIC EVIDENCE

Single-nucleus transcriptome profiling of skeletal muscle in Diqing Tibetan pigs with distinct body sizes

BMC Genomics
Suyun Fang, Yuedong Zhang, Ruifei Yang

Publication Overview

Key findings, Countstar context, and access to the original paper.
2025
Cell Counting & Viability
Key Finding
Single-nucleus RNA sequencing compared skeletal muscle from Diqing Tibetan pigs with different body sizes. The analysis resolved muscle cell populations, transcriptional states, and pathways associated with growth-related phenotypes, providing a cell-resolved resource for understanding muscle development and body-size variation in this indigenous breed.
Countstar Connection
The Countstar Mira FL counted AO/PI- and trypan blue-stained nuclei, enabling the isolated nuclei suspension to be adjusted before single-nucleus RNA sequencing.
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