Single-cell transcriptomic analysis deciphers the inflammatory microenvironment characterized by CXCL9+ fibroblasts and ACKR1+ endothelial cells in immune-related myocarditis.

Analysis of murine scRNA-seq data further supported these findings, revealing that exhausted CD8 + T cells and pro-inflammatory fibroblasts were uniquely enriched in ICI-related myocarditis, reflecting its distinct inflammatory microenvironment. The work revealed key cell subpopulations that were significantly implicated in inflammation, thus offering potential therapeutic targets.

Within the study, Countstar Rigel S2 measured cell number and viability in the study's cell samples during preparation for single-cell or single-nucleus analysis.