Single-cell transcriptome sequencing-based analysis: probing the mechanisms of glycoprotein NMB regulation of epithelial cells involved in silicosis.

The authors elucidated the pathogenesis of pulmonary fibrosis via single-cell transcriptome sequencing and constructed an experimental silicosis mouse model to explore the specific molecular mechanisms affecting epithelial mesenchymal transition at the single-cell level. The findings suggest that GPNMB is a potential target for fibrosis prevention.

Researchers used Countstar (model not specified) to assess cell measurements in cell suspensions during preparation for single-cell or single-nucleus analysis.