Single-cell RNA sequencing reveals cellular dynamics and therapeutic effects of astragaloside IV in slow transit constipation.

AS-IV reinstated fecal butyrate levels and improved energy metabolism in epithelial cells. The status of fibroblasts in STC undergoes alterations, and the FB_C4_Adamdec1 subset, associated with angiogenesis and the Wingless-related integration (Wnt) pathway, emerges.

Cell-viability assessment in the study's cell samples used Countstar (model not specified) before single-cell analysis for the study's single-cell analysis.