Single-Cell RNA-Sequencing of Peripheral Blood Reveals Neutrophil Heterogeneity in Childhood ANCA-Associated Vasculitis.

Single-cell analysis identified seven neutrophil clusters in childhood ANCA-associated vasculitis. The N2 CD10-positive, CD11B-high population was significantly expanded, demonstrating marked circulating neutrophil heterogeneity and identifying a disease-associated subset with a distinct inflammatory profile.

Countstar (model not specified) measured cell concentration and viability in the study's cell samples during preparation for single-cell or single-nucleus analysis.