Single-cell RNA sequencing identifies endothelial-derived HBEGF as promoting pancreatic beta cell proliferation in mice via the EGFR-Kmt5a-H4K20me pathway.

Aims/hypothesis Pancreatic beta cell mass is dynamically regulated in response to increased physiological and pathological demands. The crosstalk between endothelial cells and the proliferating beta cell subcluster, mediated by the HBEGF-EGF receptor (EGFR) ligand-receptor interaction, increased as beta cell mass expanded.

Using Countstar Rigel S2, the study measured cell viability in the study's cell samples during preparation for single-cell or single-nucleus analysis.