Single-cell landscape of bronchoalveolar immune cells in patients with immune checkpoint inhibitor-related pneumonitis

Single-cell and T-cell receptor sequencing of bronchoalveolar lavage fluid revealed T-cell enrichment, inflammatory signaling, and distinct immune-cell states associated with immune checkpoint inhibitor-related pneumonitis.

A Countstar Rigel S2 cell counter was used to standardize single-cell suspension concentration before 10x Genomics single-cell RNA and T-cell receptor library preparation.