Serum-free long-term expansion and functional maintenance of pig satellite cells via targeted genetic engineering for cultured meat

Targeted genetic engineering and serum-free culture conditions were combined to support long-term expansion of porcine satellite cells for cultured meat. The engineered cells retained proliferative and differentiation capacity and were evaluated in three-dimensional constructs, addressing scalability and functional maintenance in animal-cell agriculture.

Trypan blue measurements on the Countstar Mira FL tracked cell number, viability, and expansion fold during long-term serum-free cultivation of engineered pig satellite cells.