Selective loss and transcriptional reprogramming of Nox4 + GABAergic neurons in the trigeminal nucleus caudalis of NTG-induced chronic migraine model.

This subpopulation was significantly reduced in NTG-treated mice compared with controls (p = 0.018), a finding further validated by immunofluorescence staining. hdWGCNA identified a migraine-associated co-expression module enriched in GABA_Nox4 neurons, which was further supported by disease enrichment analysis.

Using Countstar Rigel S2, the researchers measured nuclei numbers in isolated nuclei during quality assessment for the reported research.