Scalable preparation of osteogenic micro-tissues derived from hESC-derived immunity-and-matrix-regulatory cells within porous microcarriers in suspension culture.

In this study, the authors demonstrated for the first time that human embryonic stem cell-derived MSCs, also known as immunity-and-matrix-regulatory cells (IMRCs), could be inoculated into microcarriers to create osteogenic micro-tissues appropriate for scalable production in 250 mL bioreactor. Osteogenic micro-tissues generated from IMRCs-seeded microcarriers significantly increased osteocalcin levels after 21 days of differentiation in a bioreactor.

Countstar Rigel S2 was used for cell numbers measurement in the study's cell samples during quality assessment for the immune-cell research.