Protocols for measuring phosphorylation, subcellular localization, and kinase activity of Hippo pathway components YAP and LATS in cultured cells.

The authors use HEK293A cells as an example, but the protocols can be applied to other cell lines and tissues. For complete details on the use and execution of this profile, please refer to Luo et al.

Using Countstar (model not specified), the researchers measured cell numbers in the study's cell samples during quality assessment for the reported research.