Protocol to dissociate and isolate wide-diversity single cells by density gradient centrifugation from human hepatoblastoma tissue.
STAR protocols
Zhou X, Meng M, Wu Y, Gao R, Shan Y, Gu S, He J.
Publication Overview
Key findings, Countstar context, and access to the original paper.
2023
Immunology
Single-Cell Analysis
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Key Finding
The authors describe steps for tissue processing, enzymatic digestion, Percoll density gradient separation, cell lysis, cell suspension quality control, and scRNA library construction. This protocol is applicable to preparing single-cell suspensions from other human liver tissue samples.
Countstar Connection
Using Countstar Altair, the researchers measured cell concentration and viability in single-cell suspensions within the study workflow for the study's single-cell analysis.
