Protocol for quantifying hepatitis B virus transcript abundance and genomic segment distribution from single-cell RNA-seq.

This protocol enables detailed analysis of viral expression patterns and HBV-host interactions at single-cell resolution. For complete details on the use and execution of this protocol, please refer to Zhou et al.

For the study's cell samples, Countstar Rigel S2 provided cell-count and viability measurements within the study workflow in the 2025 STAR protocols study.