New mouse models for exploring renal tumor extension into the inferior vena cava.

Single-cell transcriptome sequencing, immunohistochemistry and multiplex immunohistochemistry reveal a predominance of monocytes, macrophages, and neutrophils within IVCTT, mirroring the cellular composition of the human IVCTT; however, fewer lymphocytes are observed.

The researchers assessed cell viability in the study's cell samples with Countstar (model not specified) during preparation for single-cell or single-nucleus analysis.