Multi-omics analysis reveals NNMT as a master metabolic regulator of metastasis in esophageal squamous cell carcinoma.

By integrating analyses of scRNA-seq data with metabolomics of ESCC tumor tissues and plasma samples, the authors found nicotinate and nicotinamide metabolism pathway was dysregulated in ESCC patients with lymph node metastasis (LN + ), exhibiting as significantly increased 1-methylnicotinamide (MNA) in both tumors and plasma.

The study's cell samples underwent cell-concentration and viability assessment with Countstar (model not specified) during preparation for single-cell or single-nucleus analysis.