This peer-reviewed protocol describes isolation of mouse liver non-parenchymal cells and an affinity-screening workflow for identifying Kupffer-cell receptors that recognize pneumococcal capsules. It provides operational guidance for cell preparation, receptor enrichment, and downstream analysis, supporting reproducible investigation of host recognition of encapsulated Streptococcus pneumoniae.
Countstar Connection
The Countstar Mira FL fluorescence analyzer counted isolated mouse liver non-parenchymal cells before the affinity-screening workflow for Kupffer-cell receptor identification.
