SCIENTIFIC EVIDENCE

Integrating single-cell RNA and T cell/B cell receptor sequencing with mass cytometry reveals dynamic trajectories of human peripheral immune cells from birth to old age

Nature Immunology
Yufei Wang, Ronghong Li, Renyang Tong, Taiwei Chen, Mingze Sun, Lingjie Luo, Zheng Li, Yifan Chen, Yichao Zhao, Chensheng Zhang, Lin Wei, Wei Lin, Haoyan Chen, Kun Qian, Alex F. Chen, Junling Liu, Lei Chen, Bin Li, Feng Wang, Li Wang, Bing Su, Jun Pu

Publication Overview

Key findings, Countstar context, and access to the original paper.
2025
Immunology
Single-Cell Analysis
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Key Finding
A lifespan-spanning immune atlas combined single-cell RNA and receptor sequencing with mass cytometry to reveal age-associated trajectories across major peripheral immune-cell populations.
Countstar Connection
A Countstar automated cell counter was used to quantify viable PBMC suspensions at 400–600 live cells per microliter before 10x Genomics single-cell library preparation.
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