Identification of permissive amber suppression sites for efficient non-canonical amino acid incorporation in mammalian cells.
Nucleic acids research
Bartoschek MD, Ugur E, Nguyen TA, Rodschinka G, Wierer M, Lang K, Bultmann S.
Publication Overview
Key findings, Countstar context, and access to the original paper.
2021
Stem Cell Research
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Key Finding
The authors show that nucleotides up- and downstream of UAG synergistically influence ncAA incorporation efficiency independent of cell line and ncAA identity. Additionally, the authors demonstrate iPASS-guided optimization of ncAA incorporation rates by synonymous exchange of codons flanking the amber stop codon.
Countstar Connection
The researchers relied on Countstar (model not specified) to measure cell numbers in the study's cell samples during culture monitoring for the reported research.
