The authors show that nucleotides up- and downstream of UAG synergistically influence ncAA incorporation efficiency independent of cell line and ncAA identity. Additionally, the authors demonstrate iPASS-guided optimization of ncAA incorporation rates by synonymous exchange of codons flanking the amber stop codon.
Countstar Connection
The researchers relied on Countstar (model not specified) to measure cell numbers in the study's cell samples during culture monitoring for the reported research.
