Histone Trimethylations and HDAC5 Regulate Spheroid Subpopulation and Differentiation Signaling of Human Adipose-Derived Stem Cells.

During spheroid formation, the authors observed significant increases in histone 3 trimethylation at lysine 4 (H3K4me3), lysine 9 (H3K9me3), and lysine 27 (H3K27me3), accompanied by increased histone deacetylase (HDAC) activities and decreased histone acetyltransferase activities.

The research used Countstar (model not specified) for cell-concentration assessment in the study's cell samples before single-cell analysis for the organoid or 3D-culture experiments.