SCIENTIFIC EVIDENCE

Generation of miniploid cells and improved natural transformation procedure for a model cyanobacterium Synechococcus elongatus PCC 7942.

Frontiers in microbiology
Riaz S, Jiang Y, Xiao M, You D, Klepacz-Smółka A, Rasul F, Daroch M.

Publication Overview

Key findings, Countstar context, and access to the original paper.
2022
Bioprocessing
Microbiology
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Key Finding
The findings reveal that high temperature and phosphate-depleted cells contain the lowest genome copies (2.4 ± 0.3 and 1.9 ± 0.2) and showed the potential to rapidly produce fully segregated mutants. In addition, higher antibiotic concentrations improve the selection of homozygous transformants while maintaining similar genome copies at a constant temperature.
Countstar Connection
Cell numbers were assessed in the study's cell samples with a Countstar instrument during sample-quality assessment.
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