FPR1-dependent Pro-inflammatory Ccl4 high monocytes/macrophages drive and predict sepsis-induced acute lung injury.

These findings were further validated experimentally leading to the identification of tri-lineage candidate biomarkers for sepsis-associated ALI: SCGB3A2 (in epithelial cells), AKAP12 (in endothelial cells), and CCL4 (in monocytes/macrophages, Mo/Mφ). These findings enhance the study's understanding of the underlying mechanisms of sepsis-induced ALI and offer new targets for its precision diagnosis and therapeutic intervention.

In this work, Countstar Rigel S2 measured flow-cytometry cell measurements in the study's cell samples before single-cell analysis for the immune-cell research.