In this study, the authors enhanced the primary culture protocol for VCL epithelial cells and introduced simian virus 40 Large T to establish an immortalized cell line, designated hVCL-MSDEP01. The authors confirmed that hVCL-MSDEP01 expresses epithelial-specific genes and proteins; it also demonstrates distinct cell cycle dynamics and apoptosis rates compared with primary cells.
Countstar Connection
Using Countstar Rigel S2, the researchers measured cell viability in the study's cell samples before single-cell analysis for the reported research.
