Enhanced Transgene Expression by Optimization of Poly A in Transfected CHO Cells.
Frontiers in bioengineering and biotechnology
Wang XY, Du QJ, Zhang WL, Xu DH, Zhang X, Jia YL, Wang TY.
Publication Overview
Key findings, Countstar context, and access to the original paper.
2022
Bioprocessing
No items found.
Key Finding
SV40 and synthetic polyadenylation elements increased stable eGFP expression in CHO cells, reduced expression variability, and supported higher adalimumab production. The improvement was not explained by gene copy number or mRNA abundance, indicating that polyadenylation-element choice affected stable protein expression.
Countstar Connection
The research obtained cell-concentration measurements from the study's cell samples using Countstar (model not specified) during quality assessment for the reported research.
