Decoding the multicellular ecosystem of lung adenocarcinoma manifested as pulmonary subsolid nodules by single-cell RNA sequencing.

The findings show that cytotoxic natural killer/T cells were dominant in the TME of SSN, and malignant cells in SSN undergo a strong metabolic reprogram and immune stress. This resource provides deeper insight into the indolent nature of SSN and will be helpful in advancing lung cancer immunotherapy.

Researchers assessed cell concentration and viability in the study's cell samples using Countstar (model not specified) before single-cell analysis for the study's single-cell analysis.