Chemical reprogramming of fibroblasts into retinal pigment epithelium cells for vision restoration.

However, identifying key chemical compounds for generating functional RPE cells from somatic cells remains challenging. Omics and mechanistic analyses revealed that the identified compounds synergistically activate core transcription factors, including Ascl1 and Olig2, orchestrating the reprogramming process.

Using Countstar (model not specified), the study measured cell viability in the study's cell samples during preparation for single-cell or single-nucleus analysis.