An In Vitro RNA Editing-Based Reporter Assay for Transcriptional Activity of Therapeutic Gene in Gene Therapy Products.
Molecules (Basel, Switzerland)
Yu L, Zhou Y, Wang G, Fu J, Fu Z, Liang C, Wang J.
Publication Overview
Key findings, Countstar context, and access to the original paper.
2024
Immunology
Microbiology
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Key Finding
Of note, all sensors that targeted different regions, including the gene of interest, tag sequence, and 3' untranslated region, showed a dose-dependent response pattern. The sensor reporter assay, which was used for quantifying the transcriptional activity of recombinant adeno-associated virus-based gene therapy products, revealed excellent performance in terms of assay specificity, precision (inter-assay relative standard deviation 2 > 0.99).
Countstar Connection
For the study's cell samples, Countstar (model not specified) supplied flow-cytometry measurements during sample-quality assessment.
