An effective method to enhance transient gene expression by transfecting high-density CHO cells.
AMB Express
Fu YS, Han XL, Li WD, Shan LL, Geng SL, Mi CL, Dong WH, Wang TY, Wang XY.
Publication Overview
Key findings, Countstar context, and access to the original paper.
2025
Bioprocessing
Cell Counting & Viability
No items found.
Key Finding
Vector optimization led to a substantial enhancement in recombinant protein, with EGFP and SEAP levels increased by 5.33-fold and 4.13-fold, respectively (p < 0.05). In summary, high-density suspension transfection markedly boosts transient recombinant protein expression in CHO cells by improving transfection efficiency, expanding the population of recombinant cells, and maintaining high cell viability.
Countstar Connection
Using Countstar Rigel S2, the researchers measured cell number and viability in the study's cell samples during transfection assessment for the reported research.
