A rapid and accurate method for evaluating the degradation of pan-Akt in cells by PROTACs using NanoLuc luciferase.

After conducting a thorough comparison between this and the classical western blot assay in various cells, as well as testing the stability of the experiments between multiple batches, the authors found that NanoLuc luciferase is a highly accurate, stable, low-cost and easy-to-operate for the evaluation of intracellular pan-Akt degradation by PROTACs with a short cycle time and high cellular expandability.

Researchers used Countstar (model not specified) to assess cell numbers in the study's cell samples during transfection assessment.