Indlela evamile yokubala amaseli iwukubala ngesandla ku-hemocytometer.Njengathi sonke, ukubala okwenziwa ngesandla kusetshenziswa i-hemocytomter ebandakanyeka ezinyathelweni zamaphutha amaningi.Ukunemba komphumela kuncike kakhulu kulwazi nekhono labasebenzisi.I-Countstar automated cell counters ilula futhi isetshenziswa kalula, iklanyelwe ukuqeda amaphutha abangelwa isici somuntu ekubaleni ngesandla futhi inikeze umphumela wokubala wamaseli wokuzala nokunembile.
I-Countstar Automated Cell Counters Protocol
1. Hlanganisa ukumiswa kweseli ku-1:1 no-0.2 % we-trypan eluhlaza okwesibhakabhaka
2.Jonga isampula engu-20 µL kusilayidi segumbi le-Countstar.
3.Layisha isilayidi segumbi lokubala ku-Counterstar bese uhlaziya
I-Countstar iqhathaniswa kalula ne-hemocytometer
Umfanekiso A. CHO umphumela wokubala wochungechunge lwe-dilution.Imiphumela ye-Countstar ibonisa umphumela wokuzinza okuphezulu.Umfanekiso B. Ukuxhumana komphumela we-Countstar kanye ne-hemocytometer (i-CHO series dilution).
