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T/NK Cell Mediated Cytotoxicity Analysis by CFSE, Hoechest 33342 and PI
Experimental Protocol The cytotoxicity % is calculated by the equation below.Cytotoxicity % = (Live counts of control – Live counts of treated) / Live counts of control * 100 By labeling the target t...
Direct Analysis of Leukocytes in Whole Blood without Lysing
IntroductionAnalyzing the leukocytes in whole blood is a routine assay in clinical lab or blood bank. The concentration and viability of the leukocytes are the vital index as quality control of blood ...
AO PI Dual Fluorescence Analyzing the Concentration and Viability of PBMC
IntroductionPeripheral blood mononuclear cells (PBMCs) are often processed to separate from whole blood by density gradient centrifugation. Those cells are consist of lymphocytes (T cells, B cells, NK...
Cell Counting Assay
Introduction of Cell CountingThe traditional method of cell counting is by manual counting on the hemocytometer. As we all that, manual counting using a hemocytomter involved in multiple error prone s...
Monitoring the Viability of AdMSC after the Transportation
IntroductionAOPI Dual-fluoresces counting is the assay type used for detecting cell concentration and viability. The solution is a combination of acridine orange (the green-fluorescent nucleic acid st...
Determine the Immuno-phenotype of AdMSCs by Countstar FL
IntroductionImmuno-phenotyping analysis is a typical experiment performed in cell related research fields to diagnose various diseases (autoimmune disease, immunodeficiency disease, tumor diagnosis, h...
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