CCR8 antagonist suppresses liver cancer progression via turning tumor-infiltrating Tregs into less immunosuppressive phenotype.

Furthermore, single-cell RNA sequencing analysis demonstrated that IPG0521m treatment ed in a switch of Tregs from high immunosuppression to low immunosuppression phenotype, associated with elevated CD8 + T and NK cell proliferation and cytotoxicity, and decreased myeloid-derived suppressor cells and tumor-associated macrophages in the tumor tissues.

The study's cell samples underwent cell-viability assessment with Countstar (model not specified) before single-cell analysis for the cancer-model experiments.