Proteomics of embryonic stem cells and induced pluripotent stem cells

Researchers use inventory of embryonic stem cells and induced pluripotent stem cell proteome to do a comparison, found that the expression of two proteins have small differences, the new discovery could help to explain the two types of cells in differentiation for a particular type of ability difference, new results published online yesterday in the journal nature, methodology.

Like embryonic stem cells, ips cells can differentiate into various types of human cells. The use of ips stem cells, derived from adult tissue, avoids the ethical issues that arise when embryonic stem cells are used for research and medical treatment. The question, however, is whether ips cells are the exact biological equivalent of embryonic stem cells.

Joshua Coon and colleagues analyzed proteins from four human embryonic stem cell lines and four induced pluripotent cell lines using a proteomic analysis method based on a high-resolution mass spectrometer. Using reproducible analysis, they found reproducible differences in protein expression between the two types of stem cells. Their results showed that ips cells retained some of the characteristics of the differentiated cells from which they originated, which may have guided them to develop into specific cell types.
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