Progress has been made in the study of hematopoietic stem cell differentiation regulation by blood c
Recently researchers from the department of blood and cardiovascular development, institute of zoology, Chinese Academy of Sciences, in the new study resolved the Blood circulation in the early embryo developmental stage control the molecular mechanism of hematopoietic stem cell development, differentiation, the results of the study was published online on August 17, 2011, the international famous academic journals "Blood".
The corresponding author is liu feng, head of the blood and cardiovascular development research group at the institute of zoology, Chinese academy of sciences. The research was supported by the national natural science foundation of China, the ministry of science and technology and the Chinese academy of sciences.
Blood circulation is one of the most important life activities, providing oxygen and nutrients for biological organisms and maintaining physiological homeostasis. Due to the early death of the mouse model with blood circulation-related gene knockout, its role in early embryonic development, especially hematopoietic stem cell generation, is rarely studied, and the molecular mechanism is still unclear.
In this paper, the researchers applied genetics, developmental biology, molecular biology and small molecule compound processing methods, using zebrafish as the model organism, and found that blood circulation plays an important regulatory role in the programming process of hematopoietic stem cells. By studying silent heart, a genetic mutant with blood circulation defects, it was found that although the earliest hematopoietic stem cells could be produced, their subsequent fate could not be maintained, resulting in the loss of hematopoietic stem cells and the inability to differentiate into T lymphocytes. The finding was confirmed in two other mutants with circulation defects. Further research revealed that the blood circulation of stress factors, vascular specific expression of the transcription factor KLF2 in this process plays an important role. KLF2 further regulates the main genes RUNX1 and cMYB of hematopoietic stem cells by directly transcribing and regulating the expression level of nitric oxide synthase (eNOS), and ultimately affects the hematopoietic stem cell programming.
This study systematically described the molecular mechanism of blood circulation in regulating the development and differentiation of hematopoietic stem cells in the early embryonic development stage, and provided a new idea for the expansion of hematopoietic stem cells that can be used for transplantation in vitro.
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