Smart
The BioApps of the Countstar Rigel S3 System simplify routine cell lab tasks while providing high quality scientific data.
AO/PI Viability Protocol: Run two fluorescence color assays in disposable consumable to determine the percentages of live, dead cells and concentration in the presents of debris and unwanted nonnucleated cell types including red blood cells.
Affinity of Antibody: This assay run as Green color assays in FITC binding antibody to determine the affinity of the biosimilar drug.
Surface Marker Assay: Quantify specific cell populations based on surface marker expression (CD45+ CD4+, CD8 + MSC, CD56+ NK cells, etc.)
Cell Cycle: Propidium iodide (PI) is a nuclear staining dye that is frequently applied in measuring cell cycle. The protocol determining cellular DNA content in cell cycle analysis
Cell Apoptosis: Cell Apoptosis assay is a type used for determining the apoptosis percentage of cells by Annexin V-FITC/7-ADD staining method. 
GFP transfection: The green fluorescent protein (GFP) exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. This protocol can analyze counting and percentage of GFP. 
Celling Killing: Run three fluorescence color assays in disposable consumable to determine the CAR T/NK-Mediated Cytotoxicity using Tracer and Viability Dyes 
Trypan blue Protocols: Obtain cell count, viability and concentration estimations based on trypan blue staining using a disposable consumable.Three step process
The Countstar S3 System is designed to take you from sample to result in one go, simplifying your work and allowing for increased productivity and efficiency.
Reproducible and Accurate
Countstar S3 system can automatic detect 5 samples at one time, it will be more efficiency and affordable, with integrated patent “fixed focus”, the results would be more stable and reliable.
Touch Screen with Flexible Experiment Assay Management System
“App-like" interface. Allow to add multiple Experiment Assays for different operators
FCS Express Software
The optional De NovoTM FCS Express software makes graphs touchable and customizable fluorescence channel boost your experiment reach. Countstar Rigel S5 together with FCS Express is able to analyze for the cell apoptosis, cell cycle, transfection, affinity of antibody, CD marker and etc.
GMP ready
The Countstar S5 system is designed to meet the modern pharmaceutical requirements. The software complies with 21 CFR part 11. Key features include control of user access and data export, and secure audit trail. At the same time, we also supply IQ/OQ service and PQ support to help customer build a reliable system.
| Technical Specifications | |
| Model: | Countstar Rigel S5 |
| Diameter range: | 3μm ~ 180μm |
| Concentration range: | 1×104 ~ 3×107/mL |
| Objective magnification: | 5x |
| Imaging element: | 1.4 megapixel ,CCD camera |
| Excitation Light | 375nm, 480nm, 525nm |
| Emission Filter | 460nm, 535nm, 580nm, 600LP |
| USB | 1×USB 3.0 1×USB 2.0 |
| Storage: | 500GB |
| Power supply: | 110–230 V/AC, 50/60Hz |
| Screen: | 10.4 inch touchscreen |
| Weight: | 13kg (28lb) |
| Size (W X D X H): | Machine: 254×303×453mm Package size: 430×370×610mm |
| Operating temperature: | 10℃ ~ 40℃ |
| Working humidity: | 20% ~ 80% |
Dual-Fluorescence Viability
Acridine orange (AO) and Propidium iodide (PI) are nuclear nucleic acid binding dyes. The analysis excludes cell fragments, debris and artifacts particles as well as undersized events such as platelets, giving a highly accurate result. In conclusion, theCountstarsystem can be used for every step of the cell manufacturing process.
T/NK cell mediated cytotoxicity
By labeling the target tumor cells with CFSE ortransfectwith GFP. Hoechst 33342 is used for stain all cells (both T cells and tumor cells), alternatively, target tumor cells can be stained with CFSE, PI is used forstainthe dead cells (both T cells and tumor cells). This staining strategy allows for the discriminate of different cells.
Cell Apoptosis
The different phases of apoptosis are segmented into an early, middle and late stage. Specified indicators can document the single stages of apoptosis. The Countstar® Rigel S5 offers a visualized image-based solution and analyzes all of these steps with pre-loaded protocols in depth.
