New stem cell culture technique: triple growth rate
Researchers at the Massachusetts institute of technology's whitehead institute, have found a new way to increase the number of human embryonic stem cells, as well as iPS cells three times as much, without the need for mouse cells that are commonly needed in cultural method. The discoveries are published in the journal proceedings of the national academy of sciences.
Embryonic stem cells and iPS cells attract many scientists due to its outstanding ability to regenerate, these pluripotent stem cell is a new hope to many diseases, such as Parkinson's, alzheimer's disease, etc., and is also the new direction of filtering signal, at the same time, these cells can provide transplantation of cells for diabetes, nerve damage, or Other tissue damage. But these applications require millions, even ten millions of cells, while current culture methods cannot meet that demand.
In the paper, the researchers not only increased the growth rate but also tripling the number of human embryonic stem cells and iPS cells, simply by irradiation the commonly used polystyrene plates with ultraviolet light. "Polystyrene is the most common culture surface in the laboratory, and this small modification can replace the mouse feeding layer, which will have many applications.”said by Daniel Anderson, an associate professor of health sciences and technology at the harvard-mit school of technology and the author of the study.
Using mouse fibroblasts as a feeding layer is one of the key technologies for the isolation, culture and passage of embryonic stem cells, which can spread viruses or mouse macromolecules and contaminate human stem cells. In order to improve the speed of stem-cell cultures, and avoid pollution risk, the team's results efforts to explore new methods, they reported using artificial polymer matrix (containing a large number of hydrocarbons, and ester base) greatly improves the speed the growth of cells at last year's a piece of Nature Materials, although this method is very effective, it still cannot be used in mass production because these polymers are hard to synthesis into different cell culture container.
The new paper that built on previous research has helped researchers understand the best surface compounds , also by using a simple method to come up with a reasonably cost-effective way to increase the growth rate of stem cells. Researchers have adopted an innovative and unconventional methods to promote the polystyrene board concentrations of hydrocarbons and ester base: they use an ultraviolet ozone instrument (a UV ozone unit, through compiling), the researchers found that the cultured cells can grow rapidly in the absence of feeder cells after 2 1/2 minutes of UV irradiation.
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