Figure 3 Counting of aggregated cells.
A. Image of Cell sample;
B. Image of Cell Sample with identification mark by Countstar BioTech software. (Green Circle: Live cell, Yellow Circle: Dead cell, Red Circle: Aggregated Cell).
C. Aggregated Histogram
Some primary cells or subculture cells are prone to aggregate when poor culture state or excessive digestion, thus causing great difficulty in the counting of cells. With the Aggregation Calibration Function, Countstar® can realize a stimulation calculation of aggregations to ensure accurate cell counting and obtain the aggregation rate and the aggregation histogram, thus providing basis for experimenters to judge the state of cells.
Monitoring of Cell Growing
Figure 4 Cell Grow Curve.
Cell growth curve is a common method for measuring the absolute growth of cell number, an important indicator to determine cell concentration and one of the basic parameters for the culture of the basic biological properties of cells. In order to accurately describe the dynamic change in the number of cells throughout the whole process, the typical growth curve can be divided into 4 parts: incubation period with slow growth; exponential growth phase with large slope, plateau phase and decline period. The cell growth curve can be obtained by plotting the number of living cells (10’000/mL) against the culture time (h or d).