Affinity of antibody detection on Countstar Rigel
The affinity antibodies are usually measured by Elisa or Biacore, these methods are very sensitive, but they detect the antibody with the purified protein, but not natural conformation protein. Use cell immunofluorescence method, user can detect the antibody affinity with natural conformation protein. Currently, quantification of the affinity of antibody is analyzed by flow cytometry. Countstar Rigel also can provide a quick and easy way to evaluate the affinity of antibody.
Countstar Rigel can automatically capture the image and quantitative the fluorescence intensity which can reflect the antibody affinity.
Diluted the antibody into different concentrations, then incubated with the cells. The results were obtained from Countstar Rigel (both image and quantitative results)